Among the CML there is a fraction of cases (5-10%) in which the Philadelphiachromosome (Ph1) originates from variant translocations (Ph1 variant), in which there are autosomes involved other than the classic 9 and 22. Here we report a case of CML where a variant Ph1 chromosome was originated from a complex translocation involving chromosomes 3, 9 and 22 [t(3,9,22)(p21,q34,q11)]. FISH analysis suggests that the mechanism of translocation occurred in a single step. Furthermore, the RT-PCR analysis also showed the presence of the classical molecular rearrangement corresponding to the BCR/ABL1 p210 isoform. The patient showed no significant variations in the parameters of clinical response (hematologic, cytogenetic, and molecular response) from those patients carrying a standard Ph1 chromosome.

En 5-10% de los casos de Leucemia Mieloide Crónica (LMC), el cromosoma Philadelphia (Ph1) se origina como producto de translocaciones variantes (Ph1 variante). Estas últimas involucran otros autosomas además del 9 y 22 clásicamente implicados. Se presenta aquí un caso de LMC cuyo cromosoma Ph1 variante fue originado a partir de una translocación compleja que involucró los cromosomas 3, 9 y 22 [t(3;9;22)(p21,q34;q11)]. El análisis mediante FISH sugiere un mecanismo de formación en una etapa única, mientras el análisis por RT-PCR mostró la presencia del rearreglo molecular BCR/ABL1 correspondiente a la isoforma p210. El paciente no presentó variaciones significativas en los parámetros de respuesta clínica (respuesta hematológica, respuesta citogenética y respuesta molecular) respecto de aquellos pacientes con el Ph1 estándar.

The aim of this work was to characterize the cytogenetic features of the croaker Pachyurus bonariensis (Perciformes, Sciaenidae). Specimens were sampled at three points along the Parana River. Chromosome preparations were obtained by means of cellular suspension from renal tissue. For cytogenetic analysis conventional and differential C and NOR bands staining techniques were applied. We studied 19 individuals of both sexes, from which a diploid number of 48 acrocentric chromosomes was determined (NF=48). There is not evidence of chromosomal sex determination system. NOR banding showed nucleolar organizer regions in pericentromeric regions of a single pair of chromosomes and C banding showed several pericentromeric blocks.

En el presente trabajo se llevo a cabo la primer caracterizacion citogenetica de la corvina Pachyurus bonariensis (Perciformes, Sciaenidae) capturada en tres puntos de muestreo sobre el Rio Parana. Las preparaciones cromosomicas se obtuvieron mediante suspension celular, utilizando tejidos renales y fueron analizadas mediante tecnicas de coloracion convencional y diferenciales (Bandeos C y NOR). Se analizaron 19 ejemplares de ambos sexos a partir de los cuales se pudo determinar un numero cromosomico diploide de 48 cromosomas acrocentricos, sin evidencias de sistemas de determinacion cromosomica del sexo. La tecnica de coloracion argentica mostro regiones organizadoras nucleolares en un solo par de cromosomas y el bandeo C bloques heterocromaticos pericentromericos en varios cromosomas del complemento.

High concentrations of xenobiotics from urban and industrial wastes have contributed to the contamination of many aquatic environments. We used the comet assay to evaluate the genotoxic potential of water collected from the River Paraná, which receives a great deal of waste, at three points (Puerto Piray, Eldorado and Montecarlo) in the Misiones Province of Argentina. The in vivo comet assay used 40 freshwater clams (Corbicula fluminea) while the in vitro comet assay used Chinese hamster (Cricetulus griseus) K1 cell (CHO-K1) cultures with the mutagen ethyl methanesulfonate (EMS) as the positive control and phosphate buffered saline (PBS) as the negative control. Both assays showed statistically significant differences between the three sampling sites in relation to the negative control, the results of this preliminary study indicating that at these three sites water from the Paraná River presents genotoxic potential.